鍗楁搗鎵�鍦╡DNA鎶�鏈В鏋愬緇村害楸肩被緹よ惤澶氭牱鎬х爺絀舵柟闈㈠彇寰楃獊鐮?/h3>
榪戞棩錛屼腑鍥芥按浜х瀛︾爺絀墮櫌鍗楁搗姘翠駭鐮旂┒鎵�闄㈢駭鍗楁搗娓斾笟璧勬簮璋冩煡涓庤瘎浼板垱鏂板洟闃熷拰鎵�綰у崡嫻風(fēng)弽紼�嬋掑嵄鍔ㄧ墿淇濇姢鍒涙柊鍥㈤槦鍦╡DNA鎶�鏈В鏋愬緇村害楸肩被緹よ惤澶氭牱鎬х爺絀舵柟闈㈠彇寰楃獊鐮達(dá)紝璇勪及浜?jiǎn)eDNA鎶�鏈彮紺哄緇村害楸肩被澶氭牱鎬х殑鍙鎬э紝寮鴻皟浜?jiǎn)eDNA鎶�鏈厠鏈嶄紶緇熸柟娉曞湪璇勪及緋葷粺鍙戣偛澶氭牱鎬ф柟闈㈠眬闄愭�х殑浠峰�箋�傜浉鍏崇爺絀舵垚鏋滀互鈥淎dvancing fish diversity monitor in degraded marine ecosystem with environmental DNA approach: Unveiling hidden riches鈥濅負(fù)棰樺彂琛ㄥ湪鍥介檯鐢熸�佸棰嗗煙鏈熷垔銆奅cological Indicators銆嬶紙鑱斿悎鍩瑰吇鐮旂┒鐢熻拫浣╂枃涓虹涓�浣滆�咃紝鏉庢晱鍜岄檲浣滃織涓洪�氳浣滆�咃級(jí)銆?/span>
浠庡涓淮搴︾悊瑙ｉ奔綾誨鏍鋒�у浜庢繁鍏ヤ簡(jiǎn)瑙ｉ奔綾葷兢钀姐�佺淮鎶ゅ鏍鋒�ф按騫沖叿鏈夐噸瑕佹剰涔夈�備紶緇熺殑鐗╃澶氭牱鎬ц瘎浼頒富瑕佸叧娉ㄥ垎綾誨鏍鋒�э紙taxonomic diversity錛孴D錛夛紝鍗崇墿縐嶄赴瀵屽害錛屽線寰�蹇借浜?jiǎn)鐗┛U嶉棿鍥烘湁鐨勭敓鎬佸樊寮傘�備負(fù)浜?jiǎn)瑙ｅ唽櫩欎簺灞�闄愭�э紝鍔熻兘澶氭牱鎬э紙functional diversity錛孎D錛夊拰緋葷粺鍙戣偛澶氭牱鎬э紙phylogenetic diversity錛孭D錛夌瓑鎸囨爣琚紩鍏ワ紝浠ユ洿鍏ㄩ潰浜?jiǎn)瑙＞煠钀界殑鐢熸�佸姛鑳藉拰閫傚簲鐜鍙樺寲鐨勬綔鍔涖�傜劧鑰岀郴緇熷彂鑲插鏍鋒�ц瘎浼伴渶瑕侀噰闆嗙兢钀戒腑姣忎釜鐗╃鐨勯仐浼犱俊鎭紝榪欏浼犵粺鐨勯噰鏍鋒柟娉曟瀯鎴愪簡(jiǎn)閲嶅ぇ鎸?xiě)鎴樺Q屽紑鍙戠畝鍗曟槗鐢ㄧ殑澶氱淮搴﹂奔綾誨鏍鋒�х爺絀舵柟妗堟樉寰楀挨涓洪噸瑕併�?/p>
鍗楁搗鎵�鍒涙柊鍥㈤槦鏋勫緩浜?jiǎn)鍩轰簬鐜DNA錛坋DNA錛夋妧鏈殑楸肩被澶氭牱鎬у緇磋瘎浠鋒柟娉曪紝騫剁粨鍚堟嫋緗戞墜孌佃В鏋愪簡(jiǎn)鍗楁搗鍖楅儴闄嗘灦嫻峰煙楸肩被緹よ惤緇撴瀯鏃剁┖鍙樺寲鐗瑰緛浠ュ強(qiáng)涓庣幆澧冨洜瀛愮殑鍏崇郴銆傚垱鏂板洟闃熼�夋嫨鍗楁搗鍖楅儴200綾蟲(chóng)按娣變互嫻呯殑嫻峰煙浣滀負(fù)鐮旂┒鍖哄煙錛屽垎鍒簬澶忓鍜屽啲瀛ｉ噰闆嗗涓珯鐐圭殑姘存牱錛堝浘1錛夛紝騫跺悓姝ヨ繘琛屼簡(jiǎn)搴曟嫋緗戦噰鏍峰拰鐜鏁版嵁閲囬泦銆傞�氳繃eDNA瀹忔潯鐮佹妧鏈В鏋愪簡(jiǎn)楸肩被鐗╃緇勬垚錛屼粠偽澶氭牱鎬у拰尾澶氭牱鎬у眰闈㈣瘎浼頒簡(jiǎn)TD銆丗D鍜孴D銆?/p>
鍥? 鍗楁搗鍖楅儴楸肩被緹よ惤絀洪棿宸紓鍙?qiáng)涓庡湴鐞嗚窛绂讳箣闂寸殑鐩稿呄x(chóng)�?/span>
閫氳繃eDNA鏂規(guī)硶鍒嗗埆鍦ㄥ瀛ｅ拰鍐媯�(gè)嫻嬪埌338縐嶅拰308縐嶉奔綾匯�俥DNA閴村畾鐨勭墿縐嶅垎鍒崰澶忓銆佸啲瀛ｅ拰鎬諱綋搴曟嫋緗戞嫻嬪埌鐨勭墿縐嶇殑52.2%銆?7.1%鍜?4.9%錛堝浘2錛夈�傜兢钀界粨鏋勫樊寮備笌鍦扮悊璺濈鏈夎緝寮虹殑鐩稿叧鎬э紝鍖楅儴婀句笌涓滈儴澶ч檰鏋跺尯鐨勯奔綾葷兢钀藉憟鐜板嚭鏄捐憲宸紓錛堝浘1錛夈�?/p>
鍥? eDNA鍜屾嫋緗戞鑾風(fēng)殑楸肩被縐嶇被瀵規(guī)瘮
鍦ㄎ卞鏍鋒�ф柟闈紝鍗楁搗鍖楅儴澶忓鐨勯奔綾誨垎綾誨鏍鋒�э紙species錛?25 vs.102錛夈�佸姛鑳藉鏍鋒�э紙F(tuán)Ric錛?.51 vs.0.19錛変互鍙?qiáng)绯痪l熷彂鑲插鏍鋒�э紙PD錛?6.27 vs. 15.81錛夊潎楂樹(shù)簬鍐錛堝浘3錛夈�傚湪尾澶氭牱鎬ф柟闈紝鐗╃鏇挎崲錛圧eplacement錛夊鎬諱綋尾澶氭牱鎬х殑璐＄尞澶т簬涓板瘜搴﹀樊寮傦紙Richness difference錛夌殑璐＄尞錛堝浘4錛夛紝璇存槑瀛ｈ妭闂寸兢钀界粨鏋勭殑宸紓涓昏鐢辯墿縐嶆浛鎹㈤┍鍔ㄣ�傜粨鏋滆〃鏄庡崡嫻峰寳閮ㄩ奔綾誨湪澶忓鏇翠負(fù)涓板瘜鍜屾椿璺冿紝鑰屽湪鍐琛ㄧ幇鍑烘洿鏄捐憲鐨勭┖闂村垎甯冨樊寮傦紝濡傝縼縐誨埌綰害杈冧綆銆佹俯搴﹁緝楂樼殑鍦板尯榪囧啲銆傛澶栵紝鍒╃敤姘寸幆澧冨拰鍦扮悊鐜鍥犵礌瑙ｉ噴浜?jiǎn)鍗棆狗鍖楅儴楸季c晃卞拰尾澶氭牱鎬х殑鍙樺寲錛氬瀛ｉ奔綾葷兢钀界粨鏋勬洿澶氬湴鍙楀埌姘寸幆澧冨洜绱犵殑褰卞搷錛岃�屽啲瀛ｅ垯鏇村鍦板彈鍒板湴鐞嗗洜绱犵殑褰卞搷錛堝浘5錛夈�?/p>
鍥? 鍩轟簬eDNA瀹忔潯鐮佺殑鍗楁搗鍖楅儴楸肩被偽澶氭牱鎬э紙TD偽銆丗D偽鍜孭D偽錛夊鑺傚樊寮?/span>
鍥? 鍩轟簬eDNA瀹忔潯鐮佺殑鍗楁搗鍖楅儴楸肩被尾澶氭牱鎬э紙TD尾銆丗D尾鍜孭D尾錛?/span>
璇ョ爺絀跺緱鍒板浗瀹墮噸鐐圭爺鍙戣鍒掋�佷腑鍥芥按浜х瀛︾爺絀墮櫌涓ぎ綰у叕鐩婃�х鐮旈櫌鎵�鍩烘湰縐戠爺涓氬姟璐圭瓑欏圭洰璧勫姪銆?/p>
璁烘枃閾炬帴錛?a >https://www.sciencedirect.com/science/article/pii/S1470160X24003509
鍥? 姘寸幆澧冨洜瀛愮殑瀛ｈ妭宸紓涓庨奔綾誨鏍鋒�ф寚鏍囩殑鐩稿叧鎬?/span>
寰俊鎵竴鎵?br>鍒嗕韓鍒版湅鍙嬪湀
榪戞棩錛屼腑鍥芥按浜х瀛︾爺絀墮櫌鍗楁搗姘翠駭鐮旂┒鎵�闄㈢駭鍗楁搗娓斾笟璧勬簮璋冩煡涓庤瘎浼板垱鏂板洟闃熷拰鎵�綰у崡嫻風(fēng)弽紼�嬋掑嵄鍔ㄧ墿淇濇姢鍒涙柊鍥㈤槦鍦╡DNA鎶�鏈В鏋愬緇村害楸肩被緹よ惤澶氭牱鎬х爺絀舵柟闈㈠彇寰楃獊鐮達(dá)紝璇勪及浜?jiǎn)eDNA鎶�鏈彮紺哄緇村害楸肩被澶氭牱鎬х殑鍙鎬э紝寮鴻皟浜?jiǎn)eDNA鎶�鏈厠鏈嶄紶緇熸柟娉曞湪璇勪及緋葷粺鍙戣偛澶氭牱鎬ф柟闈㈠眬闄愭�х殑浠峰�箋�傜浉鍏崇爺絀舵垚鏋滀互鈥淎dvancing fish diversity monitor in degraded marine ecosystem with environmental DNA approach: Unveiling hidden riches鈥濅負(fù)棰樺彂琛ㄥ湪鍥介檯鐢熸�佸棰嗗煙鏈熷垔銆奅cological Indicators銆嬶紙鑱斿悎鍩瑰吇鐮旂┒鐢熻拫浣╂枃涓虹涓�浣滆�咃紝鏉庢晱鍜岄檲浣滃織涓洪�氳浣滆�咃級(jí)銆?/span>
浠庡涓淮搴︾悊瑙ｉ奔綾誨鏍鋒�у浜庢繁鍏ヤ簡(jiǎn)瑙ｉ奔綾葷兢钀姐�佺淮鎶ゅ鏍鋒�ф按騫沖叿鏈夐噸瑕佹剰涔夈�備紶緇熺殑鐗╃澶氭牱鎬ц瘎浼頒富瑕佸叧娉ㄥ垎綾誨鏍鋒�э紙taxonomic diversity錛孴D錛夛紝鍗崇墿縐嶄赴瀵屽害錛屽線寰�蹇借浜?jiǎn)鐗┛U嶉棿鍥烘湁鐨勭敓鎬佸樊寮傘�備負(fù)浜?jiǎn)瑙ｅ唽櫩欎簺灞�闄愭�э紝鍔熻兘澶氭牱鎬э紙functional diversity錛孎D錛夊拰緋葷粺鍙戣偛澶氭牱鎬э紙phylogenetic diversity錛孭D錛夌瓑鎸囨爣琚紩鍏ワ紝浠ユ洿鍏ㄩ潰浜?jiǎn)瑙＞煠钀界殑鐢熸�佸姛鑳藉拰閫傚簲鐜鍙樺寲鐨勬綔鍔涖�傜劧鑰岀郴緇熷彂鑲插鏍鋒�ц瘎浼伴渶瑕侀噰闆嗙兢钀戒腑姣忎釜鐗╃鐨勯仐浼犱俊鎭紝榪欏浼犵粺鐨勯噰鏍鋒柟娉曟瀯鎴愪簡(jiǎn)閲嶅ぇ鎸?xiě)鎴樺Q屽紑鍙戠畝鍗曟槗鐢ㄧ殑澶氱淮搴﹂奔綾誨鏍鋒�х爺絀舵柟妗堟樉寰楀挨涓洪噸瑕併�?/p>
鍗楁搗鎵�鍒涙柊鍥㈤槦鏋勫緩浜?jiǎn)鍩轰簬鐜DNA錛坋DNA錛夋妧鏈殑楸肩被澶氭牱鎬у緇磋瘎浠鋒柟娉曪紝騫剁粨鍚堟嫋緗戞墜孌佃В鏋愪簡(jiǎn)鍗楁搗鍖楅儴闄嗘灦嫻峰煙楸肩被緹よ惤緇撴瀯鏃剁┖鍙樺寲鐗瑰緛浠ュ強(qiáng)涓庣幆澧冨洜瀛愮殑鍏崇郴銆傚垱鏂板洟闃熼�夋嫨鍗楁搗鍖楅儴200綾蟲(chóng)按娣變互嫻呯殑嫻峰煙浣滀負(fù)鐮旂┒鍖哄煙錛屽垎鍒簬澶忓鍜屽啲瀛ｉ噰闆嗗涓珯鐐圭殑姘存牱錛堝浘1錛夛紝騫跺悓姝ヨ繘琛屼簡(jiǎn)搴曟嫋緗戦噰鏍峰拰鐜鏁版嵁閲囬泦銆傞�氳繃eDNA瀹忔潯鐮佹妧鏈В鏋愪簡(jiǎn)楸肩被鐗╃緇勬垚錛屼粠偽澶氭牱鎬у拰尾澶氭牱鎬у眰闈㈣瘎浼頒簡(jiǎn)TD銆丗D鍜孴D銆?/p>
鍥? 鍗楁搗鍖楅儴楸肩被緹よ惤絀洪棿宸紓鍙?qiáng)涓庡湴鐞嗚窛绂讳箣闂寸殑鐩稿呄x(chóng)�?/span>
閫氳繃eDNA鏂規(guī)硶鍒嗗埆鍦ㄥ瀛ｅ拰鍐媯�(gè)嫻嬪埌338縐嶅拰308縐嶉奔綾匯�俥DNA閴村畾鐨勭墿縐嶅垎鍒崰澶忓銆佸啲瀛ｅ拰鎬諱綋搴曟嫋緗戞嫻嬪埌鐨勭墿縐嶇殑52.2%銆?7.1%鍜?4.9%錛堝浘2錛夈�傜兢钀界粨鏋勫樊寮備笌鍦扮悊璺濈鏈夎緝寮虹殑鐩稿叧鎬э紝鍖楅儴婀句笌涓滈儴澶ч檰鏋跺尯鐨勯奔綾葷兢钀藉憟鐜板嚭鏄捐憲宸紓錛堝浘1錛夈�?/p>
鍥? eDNA鍜屾嫋緗戞鑾風(fēng)殑楸肩被縐嶇被瀵規(guī)瘮
鍦ㄎ卞鏍鋒�ф柟闈紝鍗楁搗鍖楅儴澶忓鐨勯奔綾誨垎綾誨鏍鋒�э紙species錛?25 vs.102錛夈�佸姛鑳藉鏍鋒�э紙F(tuán)Ric錛?.51 vs.0.19錛変互鍙?qiáng)绯痪l熷彂鑲插鏍鋒�э紙PD錛?6.27 vs. 15.81錛夊潎楂樹(shù)簬鍐錛堝浘3錛夈�傚湪尾澶氭牱鎬ф柟闈紝鐗╃鏇挎崲錛圧eplacement錛夊鎬諱綋尾澶氭牱鎬х殑璐＄尞澶т簬涓板瘜搴﹀樊寮傦紙Richness difference錛夌殑璐＄尞錛堝浘4錛夛紝璇存槑瀛ｈ妭闂寸兢钀界粨鏋勭殑宸紓涓昏鐢辯墿縐嶆浛鎹㈤┍鍔ㄣ�傜粨鏋滆〃鏄庡崡嫻峰寳閮ㄩ奔綾誨湪澶忓鏇翠負(fù)涓板瘜鍜屾椿璺冿紝鑰屽湪鍐琛ㄧ幇鍑烘洿鏄捐憲鐨勭┖闂村垎甯冨樊寮傦紝濡傝縼縐誨埌綰害杈冧綆銆佹俯搴﹁緝楂樼殑鍦板尯榪囧啲銆傛澶栵紝鍒╃敤姘寸幆澧冨拰鍦扮悊鐜鍥犵礌瑙ｉ噴浜?jiǎn)鍗棆狗鍖楅儴楸季c晃卞拰尾澶氭牱鎬х殑鍙樺寲錛氬瀛ｉ奔綾葷兢钀界粨鏋勬洿澶氬湴鍙楀埌姘寸幆澧冨洜绱犵殑褰卞搷錛岃�屽啲瀛ｅ垯鏇村鍦板彈鍒板湴鐞嗗洜绱犵殑褰卞搷錛堝浘5錛夈�?/p>
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鍥? 鍩轟簬eDNA瀹忔潯鐮佺殑鍗楁搗鍖楅儴楸肩被尾澶氭牱鎬э紙TD尾銆丗D尾鍜孭D尾錛?/span>
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璁烘枃閾炬帴錛?a >https://www.sciencedirect.com/science/article/pii/S1470160X24003509
鍥? 姘寸幆澧冨洜瀛愮殑瀛ｈ妭宸紓涓庨奔綾誨鏍鋒�ф寚鏍囩殑鐩稿叧鎬?/span>
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